Flow cytometry is a technique used to detect and measure a population of cells. In this experiment, 11 different proteins and lipids were measured at the level of individual cells for thousands of T cells in an effort to infer the causal relationships among them (i.e., their signaling network).
Phosphorylation is the most way for proteins to pass a signal along to other molecules; hence the emphasis on measuring phosphorylated molecules in this study.
The concentration of all molecules is given on the log scale:
Var1: Meaning (units)Var2: Meaning (Cat1, Cat2)
Raf: Raf phosphorylated at S259.Mek: Mek1/mek2 phosphorylated at S217/S221.Plcg: Phospholipase C-gamma phosphorylated at Y783.PIP2: Phophatidylinositol 4,5-biphosphate.PIP3: Phophatidylinositol 3,4,5-triphosphate.Erk: Erk1/erk2 phosphorylated at T202/Y204.Akt: AKT phosphorylated at S473.PKA: Phosphorylation of of protein kinase A (3 sites)PKC: Phosphorylation of of protein kinase C at S660.P38: p38 MAPK phosphorylated at T180 and Y182Jnk: Jnk phosphorylated at T183 and Y185I obtained the data from the gss package in R. The original citation is
Sachs K, Perez O, Pe’er D, Lauffenburger DA, and Nolan GP (2005). Causal protein-signaling networks derived from multiparameter single-cell data. Science, 308: 523-529.